Detection and identification of HLA antigens in dried spleens by means of enzyme linked immunosorbent assay (ELISA)

A Bishara; C Brautbar

doi:10.1016/0379-0738(90)90226-o

Detection and identification of HLA antigens in dried spleens by means of enzyme linked immunosorbent assay (ELISA)

Forensic Sci Int. 1990 Mar;45(1-2):97-105. doi: 10.1016/0379-0738(90)90226-o.

Authors

A Bishara¹, C Brautbar

Affiliation

¹ Department of Clinical Microbiology, Hadassah University Hospital, Jerusalem, Israel.

PMID: 2335337
DOI: 10.1016/0379-0738(90)90226-o

Abstract

HLA class-I and class-II antigens were detected in extracts of dry spleen tissue allowed to age from 1 to 17 months, using monomorphic anti HLA monoclonal antibodies and the ELISA technique. Using polymorphic HLA alloantisera samples of spleen tissue could be characterized and differentiated. The potential use of dried tissues and blood stains is discussed.

MeSH terms

Desiccation
Enzyme-Linked Immunosorbent Assay
HLA Antigens / analysis*
Humans
Organ Preservation
Spleen / immunology*

Substances

HLA Antigens