Abstract
Four genes of Takifugu rubripes, tentatively designated Tr1-Tr4, encoding homologs of pufferfish saxitoxin- and tetrodotoxin-binding protein, were identified by BLAST search and 3'-RACE. RT-PCR and MALDI-TOF mass spectrometry allowed the identification and discrimination of Tr isoforms from the non-toxically cultured specimens. The expression of Tr1 and Tr3 mRNAs exclusively in the liver and the presence of their products as 120-kDa plasma proteins were confirmed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Blood Proteins / chemistry*
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Blood Proteins / genetics
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Blood Proteins / metabolism
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Fish Proteins / chemistry*
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Fish Proteins / genetics
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Fish Proteins / metabolism
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Liver / chemistry
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Liver / metabolism*
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Molecular Sequence Data
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Protein Binding
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Protein Isoforms / chemistry
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Saxitoxin / metabolism
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Sequence Alignment
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Sodium Channels / chemistry*
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Sodium Channels / genetics
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Sodium Channels / metabolism
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Takifugu / blood*
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Tetrodotoxin / metabolism
Substances
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Blood Proteins
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Fish Proteins
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Protein Isoforms
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Sodium Channels
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tetrodotoxin-binding protein
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Saxitoxin
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Tetrodotoxin