Scaffold effects on osteogenic differentiation of equine mesenchymal stem cells: an in vitro comparative study

Macromol Biosci. 2013 Mar;13(3):348-55. doi: 10.1002/mabi.201200355. Epub 2013 Jan 18.

Abstract

The in vitro viability, osteogenic differentiation, and mineralization of four different equine mesenchymal stem cells (MSCs) from bone marrow, periosteum, muscle, and adipose tissue are compared, when they are cultured with different collagen-based scaffolds or with fibrin glue. The results indicate that bone marrow cells are the best source of MSCs for osteogenic differentiation, and that an electrochemically aggregated collagen gives the highest cell viability and best osteogenic differentiation among the four kinds of scaffolds studied.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / cytology
  • Animals
  • Bone Marrow Cells / cytology
  • Cell Differentiation / drug effects
  • Cell Survival / drug effects
  • Cells, Cultured
  • Collagen / chemistry
  • Collagen / pharmacology*
  • Fibrin Tissue Adhesive / chemistry
  • Fibrin Tissue Adhesive / pharmacology*
  • Horses
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / drug effects*
  • Muscle, Skeletal / cytology
  • Osteocytes / cytology
  • Osteocytes / drug effects*
  • Periosteum / cytology
  • Protein Isoforms / chemistry
  • Protein Isoforms / pharmacology
  • Tissue Engineering / methods*
  • Tissue Scaffolds

Substances

  • Fibrin Tissue Adhesive
  • Protein Isoforms
  • Collagen