Alterations in the MA and NC domains modulate phosphoinositide-dependent plasma membrane localization of the Rous sarcoma virus Gag protein

J Virol. 2013 Mar;87(6):3609-15. doi: 10.1128/JVI.03059-12. Epub 2013 Jan 16.

Abstract

Retroviral Gag proteins direct virus particle assembly from the plasma membrane (PM). Phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P(2)] plays a role in PM targeting of several retroviral Gag proteins. Here we report that depletion of intracellular PI(4,5)P(2) and phosphatidylinositol-(3,4,5)-triphosphate [PI(3,4,5)P(3)] levels impaired Rous sarcoma virus (RSV) Gag PM localization. Gag mutants deficient in nuclear trafficking were less sensitive to reduction of intracellular PI(4,5)P(2) and PI(3,4,5)P(3), suggesting a possible connection between Gag nuclear trafficking and phosphoinositide-dependent PM targeting.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Membrane / metabolism*
  • Gene Products, gag / genetics
  • Gene Products, gag / metabolism*
  • Mutant Proteins / genetics
  • Mutant Proteins / metabolism
  • Phosphatidylinositols / metabolism*
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism*
  • Protein Transport
  • Rous sarcoma virus / physiology*
  • Viral Matrix Proteins / genetics
  • Viral Matrix Proteins / metabolism*
  • Virus Assembly*

Substances

  • Gene Products, gag
  • MA protein, Rous sarcoma virus
  • Mutant Proteins
  • Phosphatidylinositols
  • Phosphoproteins
  • Viral Matrix Proteins