A solution 17O-NMR approach for observing an oxidized cysteine residue in Cu,Zn-superoxide dismutase

Chem Commun (Camb). 2013 Feb 18;49(14):1449-51. doi: 10.1039/c2cc36918d.

Abstract

Solution (17)O-NMR application to biological macromolecules is extremely limited. We describe here (17)O-NMR observation of the (17)O(2)-oxidized cysteine side chain of human Cu,Zn-superoxide dismutase in solution using selective (17)O(2) oxidation. (17)O-NMR with the aid of (17)O-labeling has wide potential to probe the environment and dynamics of oxidizable functionalities in proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cysteine / chemistry*
  • Dimerization
  • Humans
  • Isotope Labeling
  • Nuclear Magnetic Resonance, Biomolecular
  • Oxidation-Reduction
  • Oxygen Isotopes / chemistry
  • Solutions / chemistry*
  • Superoxide Dismutase / chemistry*
  • Superoxide Dismutase / metabolism

Substances

  • Oxygen Isotopes
  • Solutions
  • Superoxide Dismutase
  • Cysteine