Effect of conceptus on expression of prostaglandin F2α receptor in the porcine endometrium

Abstract

Increased synthesis of prostaglandin F(2α) (PGF(2α)) in the endometrium and conceptus during the implantation period results in elevated concentration of PGF(2α) in the uterine lumen in pregnant gilts. PGF(2α) exerts its effects through PGF(2α) receptor (PTGFR), a G-protein-coupled receptor. However, besides studies concerning the function of PTGFR in endometrial abnormalities, the role of PTGFR in the endometrium during early pregnancy has not been elucidated. Therefore, the aim of this study was: (1) to evaluate the profile of PTGFR gene and protein expression in the porcine endometrium during early pregnancy and the estrous cycle; (2) to determine if the effect of conceptus on PTGFR expression is dependent on type of endometrial cells-luminal epithelial (LE) or stromal (ST) cells; and (3) to elucidate if the putative effect of conceptus on endometrial PTGFR expression is mediated by estrogen receptor. We evaluated the expression pattern of PTGFR gene and protein in the endometrium during day 9, 11, 12, 15, and 18 of the estrous cycle and pregnancy (N = 4-6 per group). The expression of PTGFR mRNA was greater on day 18 of pregnancy and the estrous cycle (vs. days 9-15 of the estrous cycle and pregnancy, P < 0.05). Expression of PTGFR protein was approximately 10-fold upregulated in the endometrium on day 15 of pregnancy when compared with day 15 of the estrous cycle (P < 0.01). Endometrial expression of PTGFR protein increased from day 12 to 18 of pregnancy (P < 0.05). PTGFR mRNA was expressed in LE and ST cells. In a subsequent experiment, we used a coculture model in which LE cells were cultured on collagen-coated inserts placed in wells plated with ST cells. Day 11 or 15 conceptus-exposed medium (CEM) elevated expression of PTGFR mRNA (2- and 1.5-fold, respectively, P < 0.05) in LE cells cocultured with ST cells. CEM did not have an effect on PTGFR mRNA expression in ST cells. The 11-day CEM-induced increase of PTGFR mRNA was abolished by incubation of LE cells in the presence of the estrogen receptor antagonist (ICI-182,780; P < 0.01). Summarizing, the conceptus upregulated expression of PTGFR in the endometrium during the implantation period. Moreover, this study indicates that expression of PTGFR gene was elevated in LE cells of endometrium by embryonic signal of estradiol. Our results suggest para- and autocrine effects of PGF(2α) through its receptor PTGFR in the porcine endometrium, especially in luminal epithelium which is in direct contact with the conceptus during the implantation period.