Highly sensitive Raman gas spectroscopy is introduced for simultaneous real time analysis of O(2), CO(2), CH(4), and N(2) in order to elucidate the dynamics of greenhouse gases evolving from climate-sensitive ecosystems. The concentrations and fluxes of this suite of biogenic gases were quantified in the head space of a water-saturated, raised peat bog ecotron. The intact peat bog, exhibiting various degradation stages of peat and sphagnum moss, was exposed to various light regimes in order to determine important ecosystem parameters such as the maximum photosynthesis rate of the sphagnum as well as the extent of soil and plant respiration. Miniaturized Raman gas spectroscopy was proven to be an extremely versatile analytical technique that allows for onsite multigas analysis in high temporal resolution. Therefore it is an urgently needed tool for elucidation of complex biochemical processes especially in climate-sensitive ecosystems and consequently for the estimation of climate-relevant gas budgets.