Fly ash (FA) from coal-burning industries may be a potential inorganic soil amendment; the insight of its nutrient release and supply to soil may enhance their agricultural use. The study was conducted to assess the ability of fly ash (a coal fired thermal plant waste) to reduce soil fertility depletion and to study bioaccumulation of mineral nutrients in Jatropha curcas grown on soils amended with fly ash. Fly ash was amended to field soil at six rates (0, 5, 10, 20, 40, and 70 % w/w) on which J. curcas was grown. After 8 months of growth, the height of jatropha plants was significantly increased at 5 and 10 % FA-amended soil, whereas, biomass significantly increased at 5, 10, and 20 % FA-amended soil compared to control soil (0 % FA). Leaf nutrients uptake, followed by stems and roots uptake were highly affected by fly ash amendment to soil. Most of nutrients accumulation were increased up to 20 % fly ash and decreased thereafter. The results of available nutrient analysis of soil revealed that availability of nitrogen, potassium, sulfur, copper, iron, mangnese, and zinc declined significantly at higher levels of fly ash amendments, whereas, availability of phosphorus increased at these levels. However, pH, organic carbon, and available boron were not influenced significantly by fly ash amendment to soil. Microbial biomass C, N, and ratio of microbial-C to organic C were significantly reduced at 20 % fly ash and higher amounts. This study revealed that J. curcas plants could gainfully utilize the nutrients available in fly ash by subsequently amending soil.