Abstract
Non-viral gene vectors play an important role in the development of gene therapy. In this report, different hydrophobic chains were introduced into low molecular weight (LMW) PEI-based biodegradable oligomers to form a series of lipopolymers (LPs), and their structure-activity relationships were studied. Results revealed that the nine polymers can condense plasmid DNA well to form nanoparticles with appropriate sizes (120-250 nm) and positive zeta-potentials (+25-40 V). In vitro experiments were carried out and it was found that LP2 showed much higher transfection efficiency both in the presence and in the absence of serum under the polymer/DNA weight ratio of 0.8 in A549 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / metabolism
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Antineoplastic Agents / pharmacology*
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Cell Survival / drug effects
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DNA / chemistry
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DNA / metabolism
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DNA / pharmacology*
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Dose-Response Relationship, Drug
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Drug Screening Assays, Antitumor
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Genetic Vectors / chemistry
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Genetic Vectors / metabolism
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Genetic Vectors / pharmacology*
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Humans
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Molecular Structure
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Molecular Weight
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Polyethyleneimine / chemistry*
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Polyethyleneimine / metabolism
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Polymers / chemistry
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Polymers / metabolism
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Polymers / pharmacology*
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Structure-Activity Relationship
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Tumor Cells, Cultured
Substances
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Antineoplastic Agents
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Polymers
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Polyethyleneimine
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DNA