Purification of high-complexity peptide microarrays by spatially resolved array transfer to gold-coated membranes

Christopher Schirwitz; Felix F Loeffler; Thomas Felgenhauer; Volker Stadler; Alexander Nesterov-Mueller; Reiner Dahint; Frank Breitling; F Ralf Bischoff

doi:10.1002/adma.201203853

Purification of high-complexity peptide microarrays by spatially resolved array transfer to gold-coated membranes

Adv Mater. 2013 Mar 20;25(11):1598-602. doi: 10.1002/adma.201203853. Epub 2013 Jan 14.

Authors

Christopher Schirwitz¹, Felix F Loeffler, Thomas Felgenhauer, Volker Stadler, Alexander Nesterov-Mueller, Reiner Dahint, Frank Breitling, F Ralf Bischoff

Affiliation

¹ German Cancer Research Center (DKFZ), Functional Genome Analysis, Chip-based Peptide Libraries, Heidelberg, Germany. c.schirwitz@dkfz.de

PMID: 23315653
DOI: 10.1002/adma.201203853

Abstract

A method for the one-step purification of high-complexity peptide microarrays is presented. The entire peptide library is transferred from the synthesis support to a gold coated polyvinylidenfluoride (PVDF) membrane, whereby only full-length peptides covalently couple to the receptor membrane via an N-terminally added cysteine. Highly resolved peptide transfer and purification of up to 10 000 features per cm(2) is demonstrated.

Publication types

Research Support, Non-U.S. Gov't