Agrobacterium tumefaciens-mediated transformation of the soybean pathogen Phomopsis longicolla

Shuxian Li; John B Ridenour; Hun Kim; Robert L Hirsch; John C Rupe; Burton H Bluhm

doi:10.1016/j.mimet.2012.12.015

Agrobacterium tumefaciens-mediated transformation of the soybean pathogen Phomopsis longicolla

J Microbiol Methods. 2013 Mar;92(3):244-5. doi: 10.1016/j.mimet.2012.12.015. Epub 2013 Jan 7.

Authors

Shuxian Li¹, John B Ridenour, Hun Kim, Robert L Hirsch, John C Rupe, Burton H Bluhm

Affiliation

¹ Crop Genetics Research Unit, United States Department of Agriculture-Agricultural Research Service, Stoneville, MS 38776, USA.

PMID: 23305924
DOI: 10.1016/j.mimet.2012.12.015

Abstract

To facilitate functional genomics in the soybean pathogen Phomopsis longicolla, we developed a robust Agrobacterium tumefaciens-mediated transformation system that yielded 150-250 transformants per 1×10(6) conidia of P. longicolla. This first report of P. longicolla transformation provides a useful tool for insertional mutagenesis in an increasingly important pathogen of soybean.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Agrobacterium tumefaciens / genetics
Ascomycota / genetics*
Gene Transfer Techniques*
Glycine max / microbiology
Mutagenesis, Insertional / methods
Plant Diseases / microbiology
Transformation, Genetic*