Photocatalytic degradation of bacteriophages evidenced by atomic force microscopy

PLoS One. 2013;8(1):e53601. doi: 10.1371/journal.pone.0053601. Epub 2013 Jan 3.

Abstract

Methods to supply fresh water are becoming increasingly critical as the world population continues to grow. Small-diameter hazardous microbes such as viruses (20-100 nm diameter) can be filtered by size exclusion, but in this approach the filters are fouled. Thus, in our research, we are investigating an approach in which filters will be reusable. When exposed to ultraviolet (UV) illumination, titanate materials photocatalytically evolve (•)OH and O2(•-) radicals, which attack biological materials. In the proposed approach, titanate nanosheets are deposited on a substrate. Viruses adsorb on these nanosheets and degrade when exposed to UV light. Using atomic force microscopy (AFM), we image adsorbed viruses and demonstrate that they are removed by UV illumination in the presence of the nanosheets, but not in their absence.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adsorption
  • Bacteriophages / isolation & purification*
  • Catalysis
  • Cell Adhesion
  • Electrodes
  • Filtration / methods*
  • Microscopy, Atomic Force*
  • Nanoparticles / chemistry
  • Nanotechnology
  • Particle Size
  • Photochemistry
  • Surface Properties
  • Titanium / chemistry
  • Ultraviolet Rays
  • Water Microbiology*
  • Water Purification / methods*

Substances

  • Titanium

Grants and funding

This work was supported by the Laboratory Directed Research and Development (LDRD) program at Sandia National Laboratories. Sandia is a multiprogram laboratory operated by Sandia Corporation, a Lockheed-Martin Company, for the United States Department of Energy under Contract No. DEAC04-94AL85000. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.