Recovery and detection of botulinum neurotoxins from a nonporous surface

Matthew Lautenschlager; Susan E Maslanka; Paris A Paul; Suzanne R Kalb; John R Barr; Brian H Raphael

doi:10.1016/j.mimet.2012.12.019

Recovery and detection of botulinum neurotoxins from a nonporous surface

J Microbiol Methods. 2013 Mar;92(3):278-80. doi: 10.1016/j.mimet.2012.12.019. Epub 2012 Dec 29.

Authors

Matthew Lautenschlager¹, Susan E Maslanka, Paris A Paul, Suzanne R Kalb, John R Barr, Brian H Raphael

Affiliation

¹ Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.

PMID: 23280253
DOI: 10.1016/j.mimet.2012.12.019

Abstract

We describe the adaptation of a sample recovery method for botulinum neurotoxins from stainless steel. Botulinum toxin was recovered from surfaces left to dry for up to 16 h and detected by either ELISA or EndoPep mass spectrometry methods. In addition, we demonstrate that this method can be used to evaluate the efficacy of surface decontamination procedures.

Published by Elsevier B.V.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Botulinum Toxins / analysis*
Botulinum Toxins / isolation & purification*
Environmental Exposure
Enzyme-Linked Immunosorbent Assay / methods
Mass Spectrometry
Specimen Handling / methods*
Stainless Steel

Substances

Stainless Steel
Botulinum Toxins