Species-specific challenges in dog cloning

G A Kim; H J Oh; J E Park; M J Kim; E J Park; Y K Jo; G Jang; M K Kim; H J Kim; B C Lee

doi:10.1111/rda.12035

Species-specific challenges in dog cloning

Reprod Domest Anim. 2012 Dec:47 Suppl 6:80-3. doi: 10.1111/rda.12035.

Authors

G A Kim¹, H J Oh, J E Park, M J Kim, E J Park, Y K Jo, G Jang, M K Kim, H J Kim, B C Lee

Affiliation

¹ Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.

PMID: 23279471
DOI: 10.1111/rda.12035

Abstract

Somatic cell nuclear transfer (SCNT) is now an established procedure used in cloning of several species. SCNT in dogs involves multiple steps including the removal of the nuclear material, injection of a donor cell, fusion, activation of the reconstructed oocytes and finally transfer to a synchronized female recipient. There are therefore many factors that contribute to cloning efficiency. By performing a retrospective analysis of 2005-2012 published papers regarding dog cloning, we define the optimum procedure and summarize the specific feature for dog cloning.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Cloning, Organism / methods
Cloning, Organism / veterinary*
Dogs / genetics*
Dogs / physiology*
Embryo Transfer
Nuclear Transfer Techniques / veterinary*
Oocytes
Species Specificity