Real-Time PCR assays for quantification of qnr genes in environmental water samples and chicken feces

Appl Environ Microbiol. 2013 Mar;79(5):1743-5. doi: 10.1128/AEM.03409-12. Epub 2012 Dec 28.

Abstract

Real-time PCR assays were developed for the enumeration of plasmid-mediated quinolone resistance (PMQR) determinants, such as the qnrA, qnrB, and qnrS genes, in different water samples and chicken feces. The results indicate that the developed assays are specific and sensitive for the quantification of qnr genes in complex samples.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Chickens
  • Drug Resistance, Bacterial*
  • Feces / microbiology
  • Plasmids / analysis*
  • Quinolones / pharmacology*
  • Real-Time Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Water Microbiology*

Substances

  • Anti-Bacterial Agents
  • Quinolones