Abstract
PankoMab-GEX is a novel humanized and glycooptimized antibody, which recognizes a novel specific tumour epitope of MUC1 (TA-MUC1). The aim of this study was to evaluate PankoMab-GEX binding to a variety of ovarian cancer specimens (n=156) and to normal ovarian tissue. In addition, PankoMab-GEX staining was compared to that of the well-known anti-MUC1 antibodies HMFG-1 and 115D8. PankoMab-GEX showed positive reactivity in serous (100% of cases, mean IRS 8.23), endometrioid (95% of cases, mean IRS 6.40), mucinous (58% of cases, mean IRS 4.17), and clear cell (92% of cases, mean IRS 7.58) carcinomas. In contrast to HMFG-1, healthy ovarian tissue was not recognized by PankoMab-GEX. Staining with antibody 115D8 was increased with staging. Cytoplasmic PankoMab-GEX staining increased with tumour grade, but no correlation was found with staging. Univariate Kaplan-Meier analysis revealed a tendency of reduced survival of patients with high expression of TA-MUC1. The findings are encouraging with respect to a potential use of PankoMab-GEX as a new therapeutic antibody for the treatment of ovarian cancer patients.
MeSH terms
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Adenocarcinoma, Clear Cell / diagnosis
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Adenocarcinoma, Clear Cell / metabolism
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Adenocarcinoma, Clear Cell / pathology
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Antibodies, Monoclonal / immunology
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Antibodies, Monoclonal / metabolism*
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Antibodies, Monoclonal, Humanized / immunology
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Antibodies, Monoclonal, Humanized / metabolism
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Antibodies, Monoclonal, Murine-Derived / immunology
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Antibodies, Monoclonal, Murine-Derived / metabolism
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Antibody Specificity
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Biomarkers, Tumor / immunology
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Biomarkers, Tumor / metabolism*
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Carcinoma, Endometrioid / diagnosis
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Carcinoma, Endometrioid / metabolism
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Carcinoma, Endometrioid / pathology
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Epitopes / immunology
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Epitopes / metabolism*
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Female
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Histological Techniques / methods
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Humans
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Mucin-1 / immunology
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Mucin-1 / metabolism*
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Neoplasms, Cystic, Mucinous, and Serous / diagnosis
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Neoplasms, Cystic, Mucinous, and Serous / metabolism
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Neoplasms, Cystic, Mucinous, and Serous / pathology
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Ovarian Neoplasms / diagnosis
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Ovarian Neoplasms / metabolism*
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Ovarian Neoplasms / pathology
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Ovary / metabolism
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Ovary / pathology
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Retrospective Studies
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Sensitivity and Specificity
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Staining and Labeling / methods*
Substances
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Antibodies, Monoclonal
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Antibodies, Monoclonal, Humanized
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Antibodies, Monoclonal, Murine-Derived
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Biomarkers, Tumor
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Epitopes
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Mucin-1
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PankoMab-GEX
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pemtumomab