¹H, ¹³C and ¹⁵N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii

Biomol NMR Assign. 2014 Apr;8(1):71-4. doi: 10.1007/s12104-012-9455-1. Epub 2012 Dec 25.

Abstract

Almost complete sequence specific (1)H, (13)C and (15)N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii are reported. The cDNA of S114A mutant of UVI31+ was cloned from a eukaryotic green algae (C. reinhardtii) and overexpressed in E.coli from where the protein was purified to homogeneity. The point mutation S114A in UVI31+ reduces its DNA endonuclease activity substantially as compared with its wild type. As a prelude to the structural characterization of S114A mutant of UVI31+, we report here complete sequence-specific (1)H, (13)C and (15)N NMR assignments.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbon Isotopes
  • Chlamydomonas reinhardtii / metabolism*
  • Hydrogen
  • Hydrogen-Ion Concentration
  • Mutant Proteins / chemistry*
  • Nitrogen Isotopes
  • Nuclear Magnetic Resonance, Biomolecular*
  • Plant Proteins / chemistry*
  • Protein Structure, Secondary

Substances

  • Carbon Isotopes
  • Mutant Proteins
  • Nitrogen Isotopes
  • Plant Proteins
  • Hydrogen