Diagnosis of albuminuria by tryptic digestion and matrix-assisted laser desorption ionization/time-of-flight mass spectrometry

Yi-Tzu Cho; Chien-Wen Chen; Miao-Pei Chen; Jia-Lun Hu; Hung Su; Jentaie Shiea; Wen-Jeng Wu; Deng-Chyang Wu

doi:10.1016/j.cca.2012.12.016

Diagnosis of albuminuria by tryptic digestion and matrix-assisted laser desorption ionization/time-of-flight mass spectrometry

Clin Chim Acta. 2013 May:420:76-81. doi: 10.1016/j.cca.2012.12.016. Epub 2012 Dec 22.

Authors

Yi-Tzu Cho¹, Chien-Wen Chen, Miao-Pei Chen, Jia-Lun Hu, Hung Su, Jentaie Shiea, Wen-Jeng Wu, Deng-Chyang Wu

Affiliation

¹ Department of Cosmetic Applications and Management, Yuh-Ing Junior College of Health Care & Management, Kaohsiung, Taiwan.

PMID: 23266772
DOI: 10.1016/j.cca.2012.12.016

Abstract

Background: Matrix-assisted laser desorption ionization/time-of-flight (MALDI-TOF) mass spectrometry has been successfully used to detect trace albumin in urine for the diagnosis of albuminuria. However, only the monomeric form of albumin was detected with this approach.

Methods: Trypsin was used to digest urinary albumin and its related compounds in urine to produce characteristic peptides. The digest solution was subsequently analyzed by MALDI-TOF MS to obtain peptide ion signals which were used as diagnostic biomarkers for albuminuria.

Results: The analytical protocol was optimized for efficient digestion and high-performance MALDI-TOF MS analysis. The limit of detection (LOD) of albumin in urine was about 5×10(-7)M.

Conclusions: Trypsin digestion combined with MALDI-TOF MS analysis is an efficient and simple approach for rapidly diagnosing albuminuria.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Albumins / metabolism*
Albuminuria / diagnosis*
Humans
Limit of Detection
Peptides / urine
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*
Trypsin / metabolism*

Substances

Albumins
Peptides
Trypsin