A comparison of Api 20A vs MALDI-TOF MS for routine identification of clinically significant anaerobic bacterial strains to the species level

Marta Kierzkowska; Anna Majewska; Robert T Kuthan; Anna Sawicka-Grzelak; Grażyna Młynarczyk

doi:10.1016/j.mimet.2012.12.007

A comparison of Api 20A vs MALDI-TOF MS for routine identification of clinically significant anaerobic bacterial strains to the species level

J Microbiol Methods. 2013 Feb 15;92(2):209-12. doi: 10.1016/j.mimet.2012.12.007. Epub 2012 Dec 20.

Authors

Marta Kierzkowska¹, Anna Majewska, Robert T Kuthan, Anna Sawicka-Grzelak, Grażyna Młynarczyk

Affiliation

¹ Chair and Department of Medical Microbiology, The Medical University of Warsaw, 5 Chalubinski Str., 02-004 Warsaw, Poland. marta.kierzkowska@wum.edu.pl

PMID: 23262031
DOI: 10.1016/j.mimet.2012.12.007

Abstract

Adequate identification of anaerobic bacteria still presents a challenge for laboratories conducting microbiological diagnostics. The aim of this study was to compare the use of Api 20A and MALDI-TOF MS techniques for identification of obligate anaerobes. The results indicate that MALDI-TOF MS ensures a rapid and accurate identification of the species isolated from patients.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Bacteria, Anaerobic / chemistry
Bacteria, Anaerobic / classification*
Bacteria, Anaerobic / isolation & purification*
Bacteria, Anaerobic / metabolism
Bacterial Infections / diagnosis*
Bacterial Typing Techniques / methods*
Humans
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Time Factors