Abstract
Canine distemper virus (CDV) was shown to use dog nectin-4 as a receptor to gain entry into epithelial cells. RNA from dog placenta or MDCK kidney cells was isolated and cDNAs were prepared. Two splice variants of dog nectin-4 were identified. A deletion of 25 amino acids was found in the cytoplasmic domain of dog nectin-4 from MDCK cells, corresponding to a splice variant that is also seen in murine nectin-4, and did not affect its role as a receptor. Both dog nectin-4 and human nectin-4 could function as an entry factor for CDV containing an EGFP reporter gene. Inhibition of dog nectin-4 expression by RNAi or nectin-4 antibodies decreased CDV titers and EGFP fluorescence. Finally, dog nectin-4 also promotes syncytia formation, which could be inhibited by siRNA treatment. These data confirm that dog nectin-4 can be used by CDV to gain entry into epithelial cells, and facilitate virus spread.
Copyright © 2012 Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies / immunology
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Cell Adhesion Molecules / genetics
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Cell Adhesion Molecules / immunology
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Cell Adhesion Molecules / metabolism*
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Chlorocebus aethiops
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Distemper Virus, Canine / genetics
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Distemper Virus, Canine / immunology
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Distemper Virus, Canine / physiology*
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Dogs
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Epithelial Cells / virology*
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Female
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Giant Cells / metabolism*
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Giant Cells / virology
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Green Fluorescent Proteins / genetics
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Humans
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MCF-7 Cells
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Madin Darby Canine Kidney Cells
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Molecular Sequence Data
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Nectins
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Placenta
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Pregnancy
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Protein Isoforms / genetics
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RNA Interference
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RNA, Small Interfering
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Receptors, Virus
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Sequence Deletion
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Vero Cells
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Virus Internalization*
Substances
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Antibodies
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Cell Adhesion Molecules
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Nectins
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Protein Isoforms
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RNA, Small Interfering
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Receptors, Virus
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enhanced green fluorescent protein
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Green Fluorescent Proteins
Associated data
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GENBANK/JX629803
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GENBANK/JX629804
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GENBANK/KC165685
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GENBANK/KC165686