Influence of environmental conditions on the expression and the maturation process of the Clostridium difficile surface associated protease Cwp84

Diana Chapetón Montes; Anne Collignon; Claire Janoir

doi:10.1016/j.anaerobe.2012.12.004

Influence of environmental conditions on the expression and the maturation process of the Clostridium difficile surface associated protease Cwp84

Anaerobe. 2013 Feb:19:79-82. doi: 10.1016/j.anaerobe.2012.12.004. Epub 2012 Dec 17.

Authors

Diana Chapetón Montes¹, Anne Collignon, Claire Janoir

Affiliation

¹ EA 4043, Université Paris-Sud 11, Faculté de Pharmacie, 5 rue Jean Baptiste Clément, 92296 Châtenay-Malabry, France.

PMID: 23257307
DOI: 10.1016/j.anaerobe.2012.12.004

Abstract

Expression of the Clostridium difficile protease gene, cwp84, was moderately up-regulated by decreasing pH due to glucose metabolism. Purification under different pH conditions influenced the proteolytic process of Cwp84. Given this, acidic pH could favor the appearance of different forms of Cwp84 that may have different roles during the infection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Clostridioides difficile / enzymology*
Clostridioides difficile / genetics*
Culture Media / chemistry
Cysteine Endopeptidases / biosynthesis*
Gene Expression Regulation, Bacterial*
Humans
Hydrogen-Ion Concentration

Substances

Culture Media
Cwp84 protein, Clostridium difficile
Cysteine Endopeptidases