Quantum dot-DNA origami binding: a single particle, 3D, real-time tracking study

Kan Du; Seung Hyeon Ko; Gregg M Gallatin; Heayoung P Yoon; J Alexander Liddle; Andrew J Berglund

doi:10.1039/c2cc37517f

Quantum dot-DNA origami binding: a single particle, 3D, real-time tracking study

Chem Commun (Camb). 2013 Jan 30;49(9):907-9. doi: 10.1039/c2cc37517f.

Authors

Kan Du¹, Seung Hyeon Ko, Gregg M Gallatin, Heayoung P Yoon, J Alexander Liddle, Andrew J Berglund

Affiliation

¹ Center for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.

PMID: 23247801
DOI: 10.1039/c2cc37517f

Abstract

The binding process of quantum dots and DNA origami was monitored using a 3D, real-time, single-particle tracking system. Single-molecule binding events were directly observed and precise measurements of the diffusion coefficient and second-order photon correlation function, g(2)(τ), were combined to distinguish free quantum dots from different conjugates of nQdot-origami.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Binding Sites
DNA / chemistry*
Diffusion
Nucleic Acid Conformation
Quantum Dots*

Substances

DNA