Enhanced transgene expression from chromatinized plasmid DNA in mouse liver

Hiroyuki Kamiya; Shiho Miyamoto; Hitomi Goto; Genki N Kanda; Miwako Kobayashi; Ichiro Matsuoka; Hideyoshi Harashima

doi:10.1016/j.ijpharm.2012.12.004

Enhanced transgene expression from chromatinized plasmid DNA in mouse liver

Int J Pharm. 2013 Jan 30;441(1-2):146-50. doi: 10.1016/j.ijpharm.2012.12.004. Epub 2012 Dec 12.

Authors

Hiroyuki Kamiya¹, Shiho Miyamoto, Hitomi Goto, Genki N Kanda, Miwako Kobayashi, Ichiro Matsuoka, Hideyoshi Harashima

Affiliation

¹ Graduate School of Science and Engineering, Ehime University, Matsuyama 790-8577, Japan. hirokam@ehime-u.ac.jp

PMID: 23247018
DOI: 10.1016/j.ijpharm.2012.12.004

Abstract

Plasmid DNA was chromatinized with core histones (H2A, H2B, H3, and H4) in vitro and was delivered into mouse liver by hydrodynamics-based administration. Transgene expression from the chromatinized plasmid DNA was more efficient than that from plasmid DNA delivered in the naked form. The use of acetylation-enriched histones isolated from cells treated with a histone deacetylase inhibitor (trichostatin A) seemed to be more effective. These results indicated that chromatinized plasmid DNA is useful for efficient transgene expression in vivo.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chromatin / chemistry
DNA / administration & dosage*
Female
Gene Expression Regulation
Histone Deacetylase Inhibitors / pharmacology
Histones / metabolism*
Hydroxamic Acids / pharmacology
Liver / metabolism*
Mice
Mice, Inbred BALB C
Plasmids
Transgenes*

Substances

Chromatin
Histone Deacetylase Inhibitors
Histones
Hydroxamic Acids
trichostatin A
DNA