The simultaneous quantification of Ganoderma acids and alcohols using ultra high-performance liquid chromatography-mass spectrometry in dynamic selected reaction monitoring mode

Abstract

Ultra-high-performance liquid chromatography-triple quadrupole mass spectrometry with dynamic selected reaction monitoring mode was developed to simultaneously quantify Ganoderma acids and alcohols. Cholic acid and hydrocortisone, whose physiochemical properties are similar to triterpene acids and alcohols, respectively, were added as internal standards before sample treatment. The method could be applied to the quantification of triterpenes in single fungus and traditional Chinese medicine preparations. This method also detected 5 triterpenes in the Coriolus genus for the first time.