A kinetic method for expeditious detection of pyrophosphate anions at nanomolar concentrations based on a nucleic acid fluorescent sensor

Chem Commun (Camb). 2013 Jan 28;49(8):798-800. doi: 10.1039/c2cc38020j.

Abstract

A kinetic method has been developed for highly sensitive and selective detection of pyrophosphate anions (PPi) by using a fluorophore labelled single-stranded-DNA-Al(III) complex system. The facile and spectrum-tunable fluorescent sensor enables rapid detection of PPi in urine and cell lysate solutions without the need for complex pre-cleanup procedures.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aluminum / chemistry
  • Anions / analysis*
  • Anions / urine
  • Cell Line
  • Coordination Complexes / chemistry
  • DNA, Single-Stranded / chemistry*
  • Diphosphates / analysis*
  • Diphosphates / urine
  • Fluorescent Dyes / chemistry*
  • Humans
  • Kinetics
  • Sensitivity and Specificity
  • Spectrometry, Fluorescence / methods

Substances

  • Anions
  • Coordination Complexes
  • DNA, Single-Stranded
  • Diphosphates
  • Fluorescent Dyes
  • diphosphoric acid
  • Aluminum