Cloning, heterologous expression, and enzymatic characterization of cathepsin L from starfish (Asterina pectinifera)

Biosci Biotechnol Biochem. 2012;76(12):2342-6. doi: 10.1271/bbb.120568. Epub 2012 Dec 7.

Abstract

To determine the role of cathepsin L in Echinoderms, starfish (Asterina pectinifera) cathepsin L (ApCtL) was cloned. The results of RT-PCR analysis indicated that the expression of ApCtL in all of the tissues. The pro-mature enzyme of ApCtL, proApCtL, was expressed in Escherichia coli, and cathepsin activity was detected by cleaving of synthetic fluorogenic peptide substrates and gelatin zymography.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Asterina / enzymology*
  • Asterina / genetics
  • Base Sequence
  • Cathepsin L / chemistry
  • Cathepsin L / genetics*
  • Cathepsin L / metabolism*
  • Cloning, Molecular
  • Gene Expression
  • Humans
  • Mice
  • Molecular Sequence Data

Substances

  • Cathepsin L

Associated data

  • GENBANK/JQ991013