Two novel SNPs of the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene associated with growth and meat quality traits in the chicken

Genet Mol Res. 2012 Sep 18;11(4):4765-74. doi: 10.4238/2012.November.12.10.

Abstract

The enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) is rate-limiting for metabolism of cholesterol; it plays an important role in endogenous cholesterol biosynthesis. We used DNA sequencing technology and created restriction site PCR-RFLP to detect HMGCR SNPs in an F(2) resource population of Gushi chicken and Anka broilers. We found a G/T mutation (Gln/His) in exon 17 and a T/C mutation (Pro/Pro) in exon 18. Based on association analysis of these HMGCR polymorphisms in 864 Gushi/Anka F(2) hybrids, these two mutations have significant effects on growth, carcass, meat quality, and lipid concentration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Avian Proteins / genetics*
  • Body Weight / genetics
  • Chickens / genetics*
  • Chickens / growth & development
  • Food Quality*
  • Gene Frequency
  • Genetic Association Studies / veterinary
  • Genetics, Population
  • Heterozygote
  • Hydroxymethylglutaryl CoA Reductases / genetics*
  • Lipid Metabolism
  • Meat / standards*
  • Polymorphism, Restriction Fragment Length
  • Polymorphism, Single Nucleotide*

Substances

  • Avian Proteins
  • Hydroxymethylglutaryl CoA Reductases