Simple colorimetric bacterial detection and high-throughput drug screening based on a graphene-enzyme complex

Juan Li; Ling-Jie Wu; Shan-Shan Guo; Hua-E Fu; Guo-Nan Chen; Huang-Hao Yang

doi:10.1039/c2nr32704j

Simple colorimetric bacterial detection and high-throughput drug screening based on a graphene-enzyme complex

Nanoscale. 2013 Jan 21;5(2):619-23. doi: 10.1039/c2nr32704j. Epub 2012 Dec 4.

Authors

Juan Li¹, Ling-Jie Wu, Shan-Shan Guo, Hua-E Fu, Guo-Nan Chen, Huang-Hao Yang

Affiliation

¹ The Key Lab of Analysis and Detection Technology for Food Safety of the MOE, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350108, PR China.

PMID: 23208411
DOI: 10.1039/c2nr32704j

Abstract

A simple, colorimetric, sensitive, cost-effective and high-throughput system based on a positively charged graphene oxide-enzyme complex was developed for bacterial detection and drug screening.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anions
Anti-Infective Agents / pharmacology
Bacteria / metabolism
Carbodiimides / chemistry
Colorimetry / methods*
Cost-Benefit Analysis
Drug Evaluation, Preclinical / methods*
Electrochemistry / methods
Environmental Monitoring / methods
Escherichia coli / enzymology
Escherichia coli / metabolism
Fluorescent Dyes / pharmacology
Graphite / chemistry*
High-Throughput Screening Assays
Microscopy, Atomic Force / methods
Models, Chemical
Multienzyme Complexes
Oxides / chemistry*
Spectrophotometry / methods
beta-Galactosidase / metabolism

Substances

Anions
Anti-Infective Agents
Carbodiimides
Fluorescent Dyes
Multienzyme Complexes
Oxides
Graphite
beta-Galactosidase