A fixed-bias spherical Langmuir probe is included as part of the Vector Electric Field Instrument (VEFI) suite on the Communication/Navigation Outage Forecast System (C/NOFS) satellite. C/NOFS gathers data in the equatorial ionosphere between 400 and 860 km, where the primary constituent ions are H(+) and O(+). The ion current collected by the probe surface per unit plasma density is found to be a strong function of ion composition. The calibration of the collected current to an absolute density is discussed, and the performance of the spherical probe is compared to other in situ instruments on board the C/NOFS satellite. The application of the calibration is discussed with respect to future fixed-bias probes; in particular, it is demonstrated that some density fluctuations will be suppressed in the collected current if the plasma composition rapidly changes along with density. This is illustrated in the observation of plasma density enhancements on C/NOFS.