Isolation of Prunin from the fruit shell of Bixa orellana and the effect of β-cyclodextrin on its binding with calf thymus DNA

Sameena Yousuf; N Sudha; G Murugesan; Israel V M V Enoch

doi:10.1016/j.carres.2012.10.003

Isolation of Prunin from the fruit shell of Bixa orellana and the effect of β-cyclodextrin on its binding with calf thymus DNA

Carbohydr Res. 2013 Jan 10:365:46-51. doi: 10.1016/j.carres.2012.10.003. Epub 2012 Oct 12.

Authors

Sameena Yousuf¹, N Sudha, G Murugesan, Israel V M V Enoch

Affiliation

¹ Department of Chemistry, Karunya University, Coimbatore 641114, India.

PMID: 23202537
DOI: 10.1016/j.carres.2012.10.003

Abstract

Naringenin-7-O-glucoside [Prunin (Pru)] was isolated from the fruit shell of Bixa orellana L. The binding of Pru with calf thymus DNA (ctDNA) and the influence of cyclomaltoheptaose (β-cyclodextrin, β-CD) on the binding were studied by absorption and fluorescence spectroscopic techniques. The comparison of the binding modes of Pru/β-CD and ctDNA-Pru/β-CD suggested that β-CD extracted Pru from DNA for forming inclusion complex. Molecular modeling gave added support to the above results. Fluorescence microscopy was used to visualize the effect of β-CD on the bindings.

MeSH terms

Animals
Binding Sites
Binding, Competitive*
Bixaceae / chemistry*
DNA / chemistry*
Fruit / chemistry*
Indicators and Reagents / chemistry
Macromolecular Substances / chemistry
Methylene Blue / chemistry
Microscopy, Fluorescence / methods
Models, Molecular
Phlorhizin / analogs & derivatives*
Phlorhizin / chemistry
Phlorhizin / isolation & purification
Plants, Medicinal / chemistry
Spectrometry, Fluorescence
beta-Cyclodextrins / chemistry*

Substances

Indicators and Reagents
Macromolecular Substances
beta-Cyclodextrins
prunin
DNA
calf thymus DNA
Phlorhizin
betadex
Methylene Blue