Abstract
By applying metabolic control analysis and inhibitor titration we determined the degree of control (flux control coefficient) of pyruvate:ferredoxin oxidoreductase (PFOR) and bifunctional aldehyde-alcohol dehydrogenase (ADHE) over the fluxes of fermentative glycolysis of Entamoeba histolytica subjected to aerobic conditions. The flux-control coefficients towards ethanol and acetate formation determined for PFOR titrated with diphenyleneiodonium were 0.07 and 0.09, whereas for ADHE titrated with disulfiram were 0.33 and -0.19, respectively. ADHE inhibition induced significant accumulation of glycolytic intermediates and lower ATP content. These results indicate that ADHE exerts significant flux-control on the carbon end-product formation of amoebas subjected to aerobic conditions.
Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetic Acid / metabolism*
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Aerobiosis
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Alcohol Dehydrogenase / antagonists & inhibitors
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Alcohol Dehydrogenase / metabolism*
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Aldehyde Oxidoreductases / antagonists & inhibitors
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Aldehyde Oxidoreductases / metabolism*
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Disulfiram / pharmacology
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Entamoeba histolytica / drug effects
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Entamoeba histolytica / metabolism*
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Enzyme Inhibitors / pharmacology
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Ethanol / metabolism
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Glycolysis / drug effects
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Kinetics
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Metabolic Networks and Pathways
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Onium Compounds / pharmacology
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Protozoan Proteins / antagonists & inhibitors
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Protozoan Proteins / metabolism*
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Pyruvate Synthase / antagonists & inhibitors
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Pyruvate Synthase / metabolism
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Trophozoites / drug effects
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Trophozoites / metabolism
Substances
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Enzyme Inhibitors
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Onium Compounds
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Protozoan Proteins
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Ethanol
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diphenyleneiodonium
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Alcohol Dehydrogenase
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Aldehyde Oxidoreductases
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Pyruvate Synthase
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Acetic Acid
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Disulfiram