Abstract
The template effect of 14-3-3 protein on the chemical ligation of fusicoccin derivatives containing an epoxide group and the pentapeptide QSYDC was investigated. HPLC analysis of the epoxide-opening reaction demonstrated that 14-3-3ζ protein improves the yield of the conjugate product compared to the protein-free control.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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14-3-3 Proteins / chemistry*
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Chemistry Techniques, Synthetic
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Chromatography, High Pressure Liquid
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Epoxy Compounds / chemistry*
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Glycosides / chemistry*
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Models, Chemical
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Models, Molecular
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Molecular Structure
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Peptide Fragments / chemistry*
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Protein Binding
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Protein Conformation
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Protein Structure, Tertiary
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
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14-3-3 Proteins
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Epoxy Compounds
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Glycosides
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Peptide Fragments
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fusicoccin