Chemical ligation of epoxide-containing fusicoccins and peptide fragments guided by 14-3-3 protein

Mol Biosyst. 2013 May;9(5):940-3. doi: 10.1039/c2mb25388g.

Abstract

The template effect of 14-3-3 protein on the chemical ligation of fusicoccin derivatives containing an epoxide group and the pentapeptide QSYDC was investigated. HPLC analysis of the epoxide-opening reaction demonstrated that 14-3-3ζ protein improves the yield of the conjugate product compared to the protein-free control.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 14-3-3 Proteins / chemistry*
  • Chemistry Techniques, Synthetic
  • Chromatography, High Pressure Liquid
  • Epoxy Compounds / chemistry*
  • Glycosides / chemistry*
  • Models, Chemical
  • Models, Molecular
  • Molecular Structure
  • Peptide Fragments / chemistry*
  • Protein Binding
  • Protein Conformation
  • Protein Structure, Tertiary
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • 14-3-3 Proteins
  • Epoxy Compounds
  • Glycosides
  • Peptide Fragments
  • fusicoccin