A novel actinomycete, strain KC-061(T) was isolated from a soil sample collected from Angthong Islands National Park, Surat Thani province, Thailand. Strain KC-061(T) was identified as a member of the genus Nonomuraea through the use of a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequencing, strain KC-061(T) belongs to the genus Nonomuraea and was closely related to 'N. monospora' PT708(T) (99.3%), N. rhizophila YIM 67092(T) (98.6%), N. dietziae DSM 44320(T) (98.5%) and N. rosea GW 12687(T) (98.3%). On the basis of phenotypic characteristics, DNA-DNA relatedness and phylogenetic distinctiveness, the novel isolate was identified as representing a novel species of the genus Nonomuraea, for which the name Nonomuraea thailandensis sp. nov. (type strain KC-061(T) =JCM 18408(T)=KCTC 29074(T) =PCU 327(T)) is proposed.