Regulating specific growth factor signaling using immobilized branched ligands

Adv Healthc Mater. 2012 Jul;1(4):457-60. doi: 10.1002/adhm.201200077. Epub 2012 May 11.

Abstract

VEGF-binding peptide ligands are incorporated into hydrogel microspheres and reduce the amount of growth factor in solution. VEGF binding affinity is enhanced by creating ligands with a dimer structure. The spheres are able to knock down VEGF-mediated HUVEC growth and reduce calcium signaling. The binding interaction is reversible, allowing the spheres to be used as a VEGF delivery vehicle.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Drug Carriers / chemistry*
  • Endothelial Cells / drug effects*
  • Endothelial Cells / metabolism*
  • Humans
  • Intercellular Signaling Peptides and Proteins / metabolism*
  • Ligands
  • Peptides / pharmacology*
  • Signal Transduction / drug effects
  • Signal Transduction / physiology*

Substances

  • Drug Carriers
  • Intercellular Signaling Peptides and Proteins
  • Ligands
  • Peptides