Abstract
Contact hypersensitivity (CHS) is an experimental model of allergic contact dermatitis (ACD) that can be studied in mice. For CHS responses, mice are immunized by painting with a reactive hapten, such as 1-fluoro-4,6-dinitrobenzene (DNFB), on the shaved abdominal and chest skin. Subsequently, the ears are challenged with diluted hapten, eliciting 'hypersensitive' ear-swelling reactions, which can be measured with a micrometer. In this manuscript we present complementary methods that can be used to evaluate CHS in mice that include: ear weight, vascular permeability, myeloperoxidase (MPO) activity, IFN-γ concentration in ear extracts and also IFN-γ production by auricular lymph node cells (ELNC). The biochemical evaluation of CHS can be also supported by proliferation assay, measurement of IFN-γ production by skin-draining lymph node cells employing ELISA test and by evaluation of IFN-γ(+) TCRαβ CD8 cells with the use of flow cytometry.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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CD8-Positive T-Lymphocytes / immunology
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CD8-Positive T-Lymphocytes / metabolism
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Capillary Permeability / immunology
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Cell Proliferation
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Dermatitis, Contact / diagnosis*
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Dermatitis, Contact / immunology*
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Dinitrofluorobenzene / immunology
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Ear, External / blood supply
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Ear, External / immunology*
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Ear, External / pathology
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Enzyme-Linked Immunosorbent Assay
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Flow Cytometry
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Haptens / immunology
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Interferon-gamma / immunology
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Interferon-gamma / metabolism
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Lymph Nodes / immunology
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Lymph Nodes / metabolism
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Lymph Nodes / pathology
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Male
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Mice
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Mice, Inbred BALB C
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Organ Size / immunology
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Peroxidase / immunology
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Peroxidase / metabolism
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Receptors, Antigen, T-Cell, alpha-beta / immunology
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Receptors, Antigen, T-Cell, alpha-beta / metabolism
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Sensitivity and Specificity
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Skin / blood supply
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Skin / immunology*
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Skin / pathology
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Specific Pathogen-Free Organisms
Substances
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Haptens
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Receptors, Antigen, T-Cell, alpha-beta
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Interferon-gamma
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Dinitrofluorobenzene
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Peroxidase