Prevalence of Providencia strains among children with diarrhea in Japan

Jpn J Infect Dis. 2012;65(6):545-7. doi: 10.7883/yoken.65.545.

Abstract

In the present study, we examined the prevalence of Providencia spp. strains among children with diarrhea in Japan. We developed a Providencia genus-specific polymerase chain reaction (PCR) method, and the specificity and sensitivity was evaluated to be 100% with various bacterial strains including 7 genera and 13 species. Five of 345 samples (1.4%) were positive by PCR using a Providencia genus-specific primer targeting the 16S rRNA gene. The single species Providencia rettgeri was isolated from 4 stool samples of children with diarrhea. The prevalence of Providencia spp. in children with diarrhea in Japan is lower than that previously reported for Japanese travelers abroad with diarrhea.

Publication types

  • Evaluation Study

MeSH terms

  • Bacteriological Techniques / methods
  • Child
  • Child, Preschool
  • DNA Primers / genetics
  • DNA, Ribosomal / genetics
  • Diarrhea / epidemiology*
  • Diarrhea / microbiology*
  • Enterobacteriaceae Infections / epidemiology*
  • Enterobacteriaceae Infections / microbiology*
  • Feces / microbiology
  • Female
  • Humans
  • Infant
  • Infant, Newborn
  • Japan / epidemiology
  • Male
  • Molecular Diagnostic Techniques / methods
  • Polymerase Chain Reaction / methods
  • Prevalence
  • Providencia / classification
  • Providencia / isolation & purification*
  • RNA, Ribosomal, 16S / genetics
  • Sensitivity and Specificity

Substances

  • DNA Primers
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S