The lichen Pseudocyphellaria coriifolia is a species endemic to southern South America. From the lichen thallus, methyl evernate, tenuiorin and three hopane triterpenoids were isolated and identified as the main lichen constituents. Their trypanocidal effects were screened against epimastigotes of Trypanosoma cruzi. The results of the biological test showed that some hopane terpenoids exhibit antiparasitic properties.