In vitro evaluation of cytotoxicity of hyaluronic acid as an extracellular matrix on OFCOL II cells by the MTT assay

Oral Surg Oral Med Oral Pathol Oral Radiol. 2014 Jun;117(6):e423-8. doi: 10.1016/j.oooo.2012.07.486. Epub 2012 Nov 10.

Abstract

Objective: To evaluate the cytotoxicity of hyaluronic acid (HA) on a tissue-engineered compound for bone grafting containing osteoblastic cells (OFCOL II), platelet-rich plasma (PRP) with or without thrombin (Thr), and hydroxyapatite (HP) by the MTT assay.

Study design: Studied groups were formed as follows: (A) Cells + HA + PRP with Thr + hydroxyapatite (HP); (B) Cells + HA + PRP + HP; (C) Cells + HA + HP; (D) Cells + HP; (E) Cells + HA; (F) Cells + PRP with Thr; (G) Cells + PRP; and (H) Pure Dulbecco's modified Eagle's medium (DMEM) with 15% fetal bovine serum. A 2-way ANOVA and Tukey's test were applied for statistical analysis (P < .05).

Results: Results of cell viability for each group were as follows: A: 79%, B: 67%, C: 68%, D: 99%, E: 74%, G: 89%, F: 90%, and Group H: 100%.

Conclusions: Results suggested a decrease in cell viability in the presence of HA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Survival / drug effects
  • Cells, Cultured
  • Durapatite / pharmacology
  • Extracellular Matrix / drug effects*
  • Humans
  • Hyaluronic Acid / toxicity*
  • In Vitro Techniques
  • Osteoblasts / drug effects*
  • Osteogenesis / drug effects
  • Platelet-Rich Plasma
  • Tetrazolium Salts / chemistry
  • Thiazoles / chemistry
  • Thrombin / pharmacology
  • Tissue Engineering

Substances

  • Tetrazolium Salts
  • Thiazoles
  • Hyaluronic Acid
  • Durapatite
  • Thrombin