Abstract
In this study, we investigated the role of PTEN (phosphatase and tensin homolog deleted on chromosome 10) in a platelet-activating factor (PAF)-induced experimental pulmonary tumor metastasis model. An adenovirus carrying PTEN cDNA (Ad-PTEN) reversed PAF-induced increase in phosphorylation of AKT as well as pulmonary metastasis of B16F10. PAF-induced pulmonary metastasis was inhibited by MAPK inhibitors, but not by PI3K inhibitor. Ad-PTEN abrogated PAF-induced phosphorylation of MAPKs. These data indicate PTEN/MAPK pathways play a key role in PAF-induced tumor metastasis.
Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Androstadienes / therapeutic use
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Animals
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Anthracenes / therapeutic use
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Butadienes / therapeutic use
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Cell Line
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Humans
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Imidazoles / therapeutic use
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Lung Neoplasms / enzymology*
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Lung Neoplasms / secondary*
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MAP Kinase Signaling System*
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Melanoma, Experimental / secondary*
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Mice
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Mice, Inbred C57BL
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Mitogen-Activated Protein Kinases / antagonists & inhibitors
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Nitriles / therapeutic use
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PTEN Phosphohydrolase / metabolism*
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Phosphoinositide-3 Kinase Inhibitors
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Phosphorylation
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Platelet Activating Factor / antagonists & inhibitors
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Platelet Activating Factor / metabolism*
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Proto-Oncogene Proteins c-akt / metabolism
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Pyridines / therapeutic use
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Wortmannin
Substances
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Androstadienes
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Anthracenes
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Butadienes
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Imidazoles
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Nitriles
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Phosphoinositide-3 Kinase Inhibitors
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Platelet Activating Factor
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Pyridines
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U 0126
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pyrazolanthrone
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Proto-Oncogene Proteins c-akt
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Mitogen-Activated Protein Kinases
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PTEN Phosphohydrolase
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Pten protein, mouse
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4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4-pyridyl)imidazole
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Wortmannin