PTEN/MAPK pathways play a key role in platelet-activating factor-induced experimental pulmonary tumor metastasis

FEBS Lett. 2012 Dec 14;586(24):4296-302. doi: 10.1016/j.febslet.2012.10.034. Epub 2012 Nov 5.

Abstract

In this study, we investigated the role of PTEN (phosphatase and tensin homolog deleted on chromosome 10) in a platelet-activating factor (PAF)-induced experimental pulmonary tumor metastasis model. An adenovirus carrying PTEN cDNA (Ad-PTEN) reversed PAF-induced increase in phosphorylation of AKT as well as pulmonary metastasis of B16F10. PAF-induced pulmonary metastasis was inhibited by MAPK inhibitors, but not by PI3K inhibitor. Ad-PTEN abrogated PAF-induced phosphorylation of MAPKs. These data indicate PTEN/MAPK pathways play a key role in PAF-induced tumor metastasis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Androstadienes / therapeutic use
  • Animals
  • Anthracenes / therapeutic use
  • Butadienes / therapeutic use
  • Cell Line
  • Humans
  • Imidazoles / therapeutic use
  • Lung Neoplasms / enzymology*
  • Lung Neoplasms / secondary*
  • MAP Kinase Signaling System*
  • Melanoma, Experimental / secondary*
  • Mice
  • Mice, Inbred C57BL
  • Mitogen-Activated Protein Kinases / antagonists & inhibitors
  • Nitriles / therapeutic use
  • PTEN Phosphohydrolase / metabolism*
  • Phosphoinositide-3 Kinase Inhibitors
  • Phosphorylation
  • Platelet Activating Factor / antagonists & inhibitors
  • Platelet Activating Factor / metabolism*
  • Proto-Oncogene Proteins c-akt / metabolism
  • Pyridines / therapeutic use
  • Wortmannin

Substances

  • Androstadienes
  • Anthracenes
  • Butadienes
  • Imidazoles
  • Nitriles
  • Phosphoinositide-3 Kinase Inhibitors
  • Platelet Activating Factor
  • Pyridines
  • U 0126
  • pyrazolanthrone
  • Proto-Oncogene Proteins c-akt
  • Mitogen-Activated Protein Kinases
  • PTEN Phosphohydrolase
  • Pten protein, mouse
  • 4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4-pyridyl)imidazole
  • Wortmannin