Heterologous expression of corn cystatin in soybean and effect on growth of the stink bug

Biosci Biotechnol Biochem. 2012;76(11):2142-5. doi: 10.1271/bbb.120432. Epub 2012 Nov 7.

Abstract

The bean bug (Riptortus clavatus) is a serious insect pest of soybean. Corn (maize) cystatin strongly inhibited the activity of its digestive cysteine proteinase. Heterologous expression of corn cystatin in soybean seeds inhibited the insect's proteases, but not its growth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animal Feed
  • Animals
  • Cystatins / chemistry
  • Cystatins / genetics*
  • Cystatins / metabolism
  • Gene Expression
  • Glycine max / genetics*
  • Hemiptera / growth & development*
  • Molecular Sequence Data
  • Pest Control, Biological / methods*
  • Plants, Genetically Modified
  • Zea mays / enzymology*
  • Zea mays / genetics*

Substances

  • Cystatins