Abstract
Glutathione (GSH) is involved in abscisic acid (ABA)- and methyl jasmonate (MeJA)-induced stomatal closure in Arabidopsis thaliana. In this study, we examined the effects of GSH-decreasing chemicals, p-nitrobenzyl chloride (PNBC), iodomethane (IDM), and ethacrynic acid (EA), on ABA- and MeJA-induced stomatal closure in Arabidopsis. Treatments with PNBC, IDM, and EA decreased GSH contents in guard cells. Depletion of GSH by PNBC and IDM enhanced ABA- and MeJA-induced stomatal closure and inhibition of light-induced stomatal opening by ABA, whereas EA did not enhance either ABA- and MeJA-induced stomatal closure or inhibition of light-induced stomatal opening by ABA. Depletion of GSH did not significantly increase the production of the reactive oxygen species (ROS), cytosolic alkalization, or cytosolic Ca(2+) oscillation induced by ABA and MeJA. These results indicate that depletion of GSH enhances ABA- and MeJA-induced stomatal closure without affecting ROS production, cytosolic alkalization, or cytosolic Ca(2+) oscillation in guard cells of Arabidopsis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Abscisic Acid / pharmacology*
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Acetates / pharmacology*
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Arabidopsis / anatomy & histology*
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Arabidopsis / cytology
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Arabidopsis / drug effects*
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Arabidopsis / radiation effects
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Calcium Signaling / drug effects
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Calcium Signaling / radiation effects
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Cyclopentanes / pharmacology*
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Cytosol / drug effects
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Cytosol / metabolism
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Cytosol / radiation effects
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Ethacrynic Acid / metabolism
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Ethacrynic Acid / pharmacology
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Glutathione / deficiency*
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Glutathione / metabolism
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Hydrocarbons, Iodinated / metabolism
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Hydrocarbons, Iodinated / pharmacology
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Light
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Nitrobenzenes / chemistry
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Nitrobenzenes / metabolism
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Nitrobenzenes / pharmacology
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Oxylipins / pharmacology*
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Plant Stomata / anatomy & histology*
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Plant Stomata / cytology
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Plant Stomata / drug effects*
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Plant Stomata / radiation effects
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Reactive Oxygen Species / metabolism
Substances
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Acetates
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Cyclopentanes
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Hydrocarbons, Iodinated
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Nitrobenzenes
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Oxylipins
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Reactive Oxygen Species
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Abscisic Acid
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methyl jasmonate
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methyl iodide
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nitrobenzene
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Glutathione
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Ethacrynic Acid