A rapid and simple method for detection of type II restriction endonucleases in cells of bacteria with high activity of nonspecific nucleases

Acta Biochim Pol. 2012;59(4):669-72. Epub 2012 Nov 6.

Abstract

In this work we describe a novel, rapid and simple microscale procedure for identification of restriction endonuclease activity in bacteria lysates, which contain high levels of non-specific DNA nucleases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / enzymology*
  • Deoxyribonucleases / chemistry
  • Deoxyribonucleases, Type II Site-Specific / chemistry
  • Deoxyribonucleases, Type II Site-Specific / isolation & purification*
  • Substrate Specificity

Substances

  • Deoxyribonucleases
  • Deoxyribonucleases, Type II Site-Specific