Abstract
We compared the mRNA expression profile of the Harmonia axyridis larvae that were either untreated or treated with LPS. The extracted mRNAs were subjected to ACP RTPCR analysis using a combination of arbitrary primers and oligo (dT) primer. Among the 47 DEGs differentially expressed, we identified a cDNA showing homology with defensin-like antibacterial peptide. The cDNA showed a putative 32-residue signal sequence and a 50-residue mature peptide named harmoniasin. We also investigated the antibacterial activity of the harmoniasin analog, which exhibited potent antibacterial activities against Gramnegative and -positive bacteria strains and it also evidenced no hemolytic activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / metabolism
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Anti-Bacterial Agents / pharmacology
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Bacteria / drug effects
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Base Sequence
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Cloning, Molecular*
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Coleoptera / chemistry
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Coleoptera / genetics*
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Coleoptera / metabolism
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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DNA, Complementary / metabolism
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Defensins / chemistry
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Defensins / genetics*
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Defensins / metabolism
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Defensins / pharmacology
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Insect Proteins / chemistry
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Insect Proteins / genetics*
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Insect Proteins / metabolism
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Insect Proteins / pharmacology
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Molecular Sequence Data
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Protein Sorting Signals
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Sequence Homology, Amino Acid
Substances
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Anti-Bacterial Agents
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DNA, Complementary
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Defensins
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Insect Proteins
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Protein Sorting Signals