Quantitative real-time PCR assay for detection of Paenibacillus polymyxa using membrane-fusion protein-based primers

J Microbiol Biotechnol. 2012 Nov;22(11):1575-9. doi: 10.4014/jmb.1207.07018.

Abstract

Paenibacillus polymyxa is known to be a plant-growthpromoting rhizobacterium. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the specific detection and quantitation of P. polymyxa using a primer pair based on the sequence of a membranefusion protein for the amplification of a 268 bp DNA fragment. This study reports that the qPCR-based method is applicable for the rapid and sensitive detection of P. polymyxa and can be used as an alternative method for agricultural soil monitoring.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • DNA Primers / genetics*
  • Membrane Fusion Proteins / genetics*
  • Membrane Fusion Proteins / metabolism
  • Paenibacillus / genetics*
  • Paenibacillus / metabolism
  • Real-Time Polymerase Chain Reaction / methods*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism

Substances

  • Bacterial Proteins
  • DNA Primers
  • Membrane Fusion Proteins
  • Recombinant Fusion Proteins