Abstract
Paenibacillus polymyxa is known to be a plant-growthpromoting rhizobacterium. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the specific detection and quantitation of P. polymyxa using a primer pair based on the sequence of a membranefusion protein for the amplification of a 268 bp DNA fragment. This study reports that the qPCR-based method is applicable for the rapid and sensitive detection of P. polymyxa and can be used as an alternative method for agricultural soil monitoring.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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DNA Primers / genetics*
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Membrane Fusion Proteins / genetics*
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Membrane Fusion Proteins / metabolism
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Paenibacillus / genetics*
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Paenibacillus / metabolism
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Real-Time Polymerase Chain Reaction / methods*
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
Substances
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Bacterial Proteins
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DNA Primers
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Membrane Fusion Proteins
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Recombinant Fusion Proteins