[Construction of TDRG1 shRNA expression vector and interfering effect of TDRG1 shRNA expression vector on NTERA-2 cells]

Shenglin Peng; Jianfu Yang; Houyang Chen; Xiaoliang Guo; Dongjie Li; Huabo Zhou; Yu Gan; Xianzhen Jiang; Yuxin Tang

doi:10.3969/j.issn.1672-7347.2012.10.002

[Construction of TDRG1 shRNA expression vector and interfering effect of TDRG1 shRNA expression vector on NTERA-2 cells]

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2012 Oct;37(10):979-82. doi: 10.3969/j.issn.1672-7347.2012.10.002.

[Article in Chinese]

Authors

Shenglin Peng¹, Jianfu Yang, Houyang Chen, Xiaoliang Guo, Dongjie Li, Huabo Zhou, Yu Gan, Xianzhen Jiang, Yuxin Tang

Affiliation

¹ Department of Urology, Third Xiangya Hospital, Central South University, Changsha, China.

PMID: 23117448
DOI: 10.3969/j.issn.1672-7347.2012.10.002

Abstract

Objective: To construct short hairpin RNA interfering expression vector of TDRG1,and detect the specific interfering effect of TDRG1-shRNA expression vector on NTERA-2 cells.

Methods: Oligos for short hairpin RNA targefing for TDRG1 were designed and connected to the expression vector pGPU6/GFP/Neo to construct the TDRG1 shRNA expression vector. The recombinant plasmid TDRG1-shRNA486, TDRG1-shRNA738, TDRG1-shRNA921 and lipofectamine ™2000 were used to generate and transfect shRNA into NTERA-2 cells. Expression of TDRG1 mRNA was assayed by RT-PCR.

Results: TDRG1-shRNA expression vector was successfully constructed. TDRG1-shRNA486 was more effective in the suppression of TDRG1 with significant reduction of TDRG1 mRNA.

Conclusion: TDRG1-shRNA can interfere the expression of TDRG1 in NTERA-2 cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Genetic Vectors*
Humans
RNA Interference*
RNA, Messenger
RNA, Small Interfering*
Transfection

Substances

RNA, Messenger
RNA, Small Interfering