Determination and analysis of ustiloxins A and B by LC-ESI-MS and HPLC in false smut balls of rice

Int J Mol Sci. 2012;13(9):11275-11287. doi: 10.3390/ijms130911275. Epub 2012 Sep 10.

Abstract

Ustiloxins are cyclopeptide mycotoxins produced by the pathogenic fungus Villosiclava virens of rice false smut. Ustiloxins A and B as two main mycotoxins were determined conveniently by LC-ESI-MS in the water extract from rice false smut balls which were mostly composed of the chlamydospores and mycelia of the pathogen. Both ustiloxins A and B in the water extract were also quantitatively analyzed by HPLC. This is the first report on the determination and analysis of ustiloxins A and B simultaneously by LC-ESI-MS and HPLC in false smut balls of rice.

Keywords: HPLC; LC-ESI-MS; Villosiclava virens; determination and analysis; false smut balls of rice; ustiloxins A and B.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ascomycota / metabolism*
  • Chromatography, High Pressure Liquid
  • Mycelium / metabolism
  • Mycotoxins / analysis
  • Oryza / microbiology*
  • Peptides, Cyclic / analysis*
  • Plant Diseases / microbiology*
  • Spectrometry, Mass, Electrospray Ionization
  • Tubulin Modulators / analysis

Substances

  • Mycotoxins
  • Peptides, Cyclic
  • Tubulin Modulators
  • ustiloxin B
  • ustiloxin A