Biolistic DNA delivery to leaf tissue of plants with the non-vacuum gene gun (HandyGun)

Anssi L Vuorinen; Arto Nieminen; Victor Gaba; Sidona Sikorskaite; Jari P T Valkonen

doi:10.1007/978-1-62703-110-3_4

Biolistic DNA delivery to leaf tissue of plants with the non-vacuum gene gun (HandyGun)

Methods Mol Biol. 2013:940:45-51. doi: 10.1007/978-1-62703-110-3_4.

Authors

Anssi L Vuorinen¹, Arto Nieminen, Victor Gaba, Sidona Sikorskaite, Jari P T Valkonen

Affiliation

¹ Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland.

PMID: 23104332
DOI: 10.1007/978-1-62703-110-3_4

Abstract

Non-vacuum gene guns such as HandyGun are flexible tools for bombardment of targets of varying size. Construction of HandyGun is simpler and cheaper than vacuum gene guns and will be described here. The conditions for maximal transient transformation efficiency of plant cells with plasmid DNA using HandyGun will be provided.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biolistics / instrumentation*
DNA / administration & dosage*
DNA / genetics*
Plant Leaves / cytology*
Plant Leaves / genetics*

Substances

DNA