Quantitative analysis of human immunodeficiency virus type-1 DNA in asymptomatic carriers using the polymerase chain reaction

S Oka; K Urayama; Y Hirabayashi; K Ohnishi; H Goto; K Mitamura; S Kimura; K Shimada

doi:10.1016/0006-291x(90)91721-4

Quantitative analysis of human immunodeficiency virus type-1 DNA in asymptomatic carriers using the polymerase chain reaction

Biochem Biophys Res Commun. 1990 Feb 28;167(1):1-8. doi: 10.1016/0006-291x(90)91721-4.

Authors

S Oka¹, K Urayama, Y Hirabayashi, K Ohnishi, H Goto, K Mitamura, S Kimura, K Shimada

Affiliation

¹ Department of Infectious Diseases, University of Tokyo, Japan.

PMID: 2310383
DOI: 10.1016/0006-291x(90)91721-4

Abstract

A method for detecting human immunodeficiency virus type 1 (HIV-1) provirus DNA in lymphocytes with improved sensitivity and reproducibility was developed using the polymerase chain reaction (PCR). Amplified HIV-1 DNA was hybridized with a 32P-labeled probe and quantitated with a beta-scanner after electrophoresis. A linear relationship was obtained between the common logarithms of the counts detected and the number of HIV-1 DNA copies applied to the PCR. Detectability was from 3 copies/10(5) lymphocytes, and linearity was maintained from 10 to 10(3) copies. HIV-1 DNA was detected in all 9 asymptomatic carriers tested (18 to 2,857 copies/10(5) CD4+ T lymphocytes). The viral burden was inversely related to the CD4+ lymphocyte count, suggesting that quantitation of provirus levels may serve as a predictor of progress in early HIV infection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Autoradiography
DNA, Viral / analysis*
Electrophoresis, Polyacrylamide Gel
Female
HIV Seropositivity / genetics*
HIV-1 / genetics*
Humans
Lymphocytes / microbiology
Male
Polymerase Chain Reaction

Substances

DNA, Viral