Stabilization and enhanced reactivity of actinorhodin polyketide synthase minimal complex in polymer-nucleotide coacervate droplets

John Crosby; Tom Treadwell; Michelle Hammerton; Konstantinos Vasilakis; Matthew P Crump; David S Williams; Stephen Mann

doi:10.1039/c2cc36533b

Stabilization and enhanced reactivity of actinorhodin polyketide synthase minimal complex in polymer-nucleotide coacervate droplets

Chem Commun (Camb). 2012 Dec 18;48(97):11832-4. doi: 10.1039/c2cc36533b.

Authors

John Crosby¹, Tom Treadwell, Michelle Hammerton, Konstantinos Vasilakis, Matthew P Crump, David S Williams, Stephen Mann

Affiliation

¹ School of Chemistry, University of Bristol, Bristol, BS8 1TS, UK. john.crosby@bristol.ac.uk

PMID: 23099989
DOI: 10.1039/c2cc36533b

Abstract

Compartmentalization of the minimal complex of actinorhodin polyketide synthase in coacervate liquid droplets produces enhanced yields of shunt polyketides under conditions of low and high ionic strength.

MeSH terms

Adenosine Triphosphate / chemistry
Adenosine Triphosphate / metabolism*
Enzyme Stability
Osmolar Concentration
Particle Size
Polyethylenes / chemistry
Polyethylenes / metabolism*
Polyketide Synthases / chemistry
Polyketide Synthases / metabolism*
Quaternary Ammonium Compounds / chemistry
Quaternary Ammonium Compounds / metabolism*
Surface Properties

Substances

Polyethylenes
Quaternary Ammonium Compounds
poly-N,N-dimethyl-N,N-diallylammonium chloride
Polyketide Synthases
Adenosine Triphosphate
polyketide beta-ketoacylsynthase