Influence of trace elements on stabilization of aqueous solutions of ascorbic acid

Biol Trace Elem Res. 2012 Dec;150(1-3):509-12. doi: 10.1007/s12011-012-9524-4. Epub 2012 Oct 26.

Abstract

Together with vitamin C, zinc, selenium, manganese, and magnesium play a vital role in the preservation of organs scheduled for transplantation. In the present study, it is shown that addition of 1 mg/l of these elements influences the stability of 0.3 mM ascorbic acid solutions. The solution's stability was estimated using an accelerated stability test. The concentration of vitamin C was measured using a validated spectrophotometric method, which uses the reduction of 2,6-dichlorophenoloindophenol by ascorbic acid. Elevated temperatures, the factor accelerating substances' decomposition reaction rate, were used in the tests. The research was conducted at two temperatures at intervals of 10 °C: 80 ± 0.1 and 90 ± 0.1 °C. It was stated that the studied substances' decomposition occurred in accordance with the equation for first-order reactions. The function of the logarithmic concentration (log%C) over time was revealed to be rectilinear. This dependence was used to determine the kinetics of decomposition reaction rate parameters. The stabilization of vitamin C solutions was measured as the time in which 10 % of the substance decomposed at 20 and 0 °C. Addition of Se(IV) or Mg(II) ions significantly increase the stability of ascorbic acid solution (∼34 and ∼16 %, respectively), but Zn(II) causes a significant decrease in stability by ∼23 %. Addition of Mn(II) has no significant influence on vitamin C stability.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / analysis
  • Antioxidants / chemistry*
  • Ascorbic Acid / analysis
  • Ascorbic Acid / chemistry*
  • Drug Stability
  • Fumarates / chemistry
  • Hot Temperature
  • Kinetics
  • Magnesium / chemistry*
  • Manganese / chemistry
  • Manganese Compounds / chemistry
  • Organ Preservation Solutions / chemistry
  • Osmolar Concentration
  • Oxidation-Reduction
  • Preservatives, Pharmaceutical / chemistry
  • Selenium / chemistry*
  • Sodium Selenite / chemistry
  • Sulfates / chemistry
  • Trace Elements / chemistry*
  • Zinc / chemistry
  • Zinc Acetate / chemistry

Substances

  • Antioxidants
  • Fumarates
  • Manganese Compounds
  • Organ Preservation Solutions
  • Preservatives, Pharmaceutical
  • Sulfates
  • Trace Elements
  • Manganese
  • fumaric acid
  • Zinc Acetate
  • Selenium
  • Sodium Selenite
  • Magnesium
  • Zinc
  • Ascorbic Acid
  • manganese sulfate